Surreal illustration of immune cells responding to Zika and Dengue viruses.

Zika vs. Dengue: Why Prior Exposure Matters for Immunity

"Decoding how past dengue infections shape the body's defense against Zika."


Zika virus (ZIKV), a mosquito-borne flavivirus, has been linked to microcephaly and severe neurological complications. Like other flaviviruses, including dengue virus (DENV), it results in substantial immunological cross-reactivity, which also means that immunity to one can affect your response to another.

Areas where dengue is common are also seeing Zika, and a key question arises: How does prior DENV exposure shape the B cell response to ZIKV? Understanding this could be a game-changer for creating vaccines and therapies that work.

Previous studies highlight the importance of three proteins in flavivirus immune responses: NS1, E, and prM. The envelope (E) protein is the main target for neutralizing antibodies. In a new study published in Science Immunology, researchers tracked how the immune system responds to Zika in people who had previously been exposed to dengue.

Dengue's Imprint: Shaping the Early Zika Response

Surreal illustration of immune cells responding to Zika and Dengue viruses.

To see how past dengue infections affect the early response to Zika, scientists followed three individuals from a dengue-prone area in Colombia who contracted Zika. For comparison, they included a Zika-infected person from the United States who had never had dengue. The main goal? To dissect the B cell response – the part of the immune system that creates antibodies to fight infections.

In those with previous dengue exposure, the acute antibody response (the plasmablast response) showed:

  • Relatively high levels of somatic hypermutation (SHM), indicating a memory B cell response.
  • A bias toward DENV binding and neutralization.
  • Evidence for original antigenic sin (OAS). This suggests that the immune system initially recalls and amplifies existing DENV-specific B cells.
In contrast, the person who had never had dengue exhibited a classical primary plasmablast response, a totally different dynamic. The bottom line? The early B cell response to ZIKV in dengue-experienced individuals is heavily influenced by their previous dengue encounters.

The Long Game: Memory B Cells and Antibody Evolution

Five months post-infection, the plot thickened. The dengue-experienced individuals developed type-specific ZIKV neutralizing antibody responses alongside DENV cross-reactive responses. These cross-reactive antibodies, however, were poorly neutralizing and sometimes enhanced ZIKV infection in lab tests.

This raises a critical concern: pre-existing DENV immunity might negatively affect protective antibody responses to ZIKV, depending on the specific epitopes targeted. It seems that the immune system's initial focus on dengue-related responses might compromise its ability to mount an effective de novo ZIKV response, at least in some individuals.

These findings underscore the need for careful ZIKV vaccine design, especially for those living in dengue-endemic regions. Vaccines should aim for ZIKV-specific epitopes to avoid skewing the immune response towards less effective, cross-reactive antibodies. It's a delicate balancing act, but understanding these immune dynamics is vital for creating effective and safe ZIKV vaccines.

About this Article -

This article was crafted using a human-AI hybrid and collaborative approach. AI assisted our team with initial drafting, research insights, identifying key questions, and image generation. Our human editors guided topic selection, defined the angle, structured the content, ensured factual accuracy and relevance, refined the tone, and conducted thorough editing to deliver helpful, high-quality information.See our About page for more information.

This article is based on research published under:

DOI-LINK: 10.1126/sciimmunol.aan6809, Alternate LINK

Title: Zika Virus Activates De Novo And Cross-Reactive Memory B Cell Responses In Dengue-Experienced Donors

Subject: General Medicine

Journal: Science Immunology

Publisher: American Association for the Advancement of Science (AAAS)

Authors: Thomas F. Rogers, Eileen C. Goodwin, Bryan Briney, Devin Sok, Nathan Beutler, Alexander Strubel, Rebecca Nedellec, Khoa Le, Michael E. Brown, Dennis R. Burton, Laura M. Walker

Published: 2017-08-04

Everything You Need To Know

1

What is Zika virus (ZIKV), and how is it related to dengue virus (DENV)?

Zika virus (ZIKV) is a mosquito-borne flavivirus, which has been associated with microcephaly and severe neurological complications. It belongs to the same family as dengue virus (DENV). Both viruses cause substantial immunological cross-reactivity, meaning that immunity to one can influence the response to the other.

2

How does prior exposure to dengue virus (DENV) affect the body's response to Zika virus (ZIKV)?

Prior exposure to DENV significantly impacts how the immune system reacts to ZIKV. Individuals with previous DENV exposure show a memory B cell response when infected with ZIKV. This involves high levels of somatic hypermutation (SHM) and a bias toward DENV binding and neutralization. This response is distinct from a primary plasmablast response observed in those without prior DENV exposure.

3

What role does the envelope (E) protein play in the context of Zika and dengue?

The envelope (E) protein is the main target for neutralizing antibodies in flaviviruses, including Zika. The study highlights the impact of original antigenic sin (OAS), where the immune system, when exposed to Zika, initially recalls and amplifies existing DENV-specific B cells. This recall response can be highly influenced by the past exposures to DENV and may lead to varied outcomes.

4

What is somatic hypermutation (SHM), and why is it significant in this context?

Somatic hypermutation (SHM) is a process where the B cells mutate their antibody genes, leading to a refined immune response. A memory B cell response, involving SHM, indicates that the immune system is quickly mobilizing pre-existing memory B cells. This contrasts with a primary response, where the body needs to generate new antibodies from scratch. In the context of ZIKV and DENV, this difference is crucial because the nature of SHM and the type of antibodies produced can determine the effectiveness of the immune response.

5

What happens to the immune response months after a Zika infection in individuals with previous dengue exposure?

Five months after infection, the dengue-experienced individuals developed type-specific ZIKV neutralizing antibody responses, but also DENV cross-reactive responses. Cross-reactive antibodies are those that can bind to both DENV and ZIKV. These antibodies, while generated, were less effective at neutralizing ZIKV, and in some instances, enhanced ZIKV infection. This shows that the long-term implications of prior DENV exposure on ZIKV immunity can be complex, potentially influencing the effectiveness of vaccines and therapies.

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