Complex network of blood cells and DNA strands representing MDS/MPN diagnostics.

Decoding MDS/MPN: How to Navigate the Complex World of Myeloid Neoplasms

"A comprehensive guide to understanding the challenges, classifications, and management of myelodysplastic/myeloproliferative neoplasms."


The landscape of chronic myeloid neoplasms spans a spectrum of conditions, from myelodysplastic syndromes (MDS), marked by ineffective hematopoiesis, to myeloproliferative neoplasms (MPN), characterized by proliferative features. Bridging these categories is a group of diseases recognized as MDS/MPN, sharing clinical, pathological, and molecular characteristics. In 2008, the World Health Organization (WHO) formally recognized this overlap, defining specific entities within this category.

Over the past decade, significant strides have been made in understanding MDS/MPN. Researchers have refined diagnostic criteria, developed risk-stratification models, and explored the underlying biology of these conditions. Treatment strategies have been adapted from those used for MDS and MPN, with ongoing efforts to define the clinical benefits of specific therapies for this unique group of patients.

This guide aims to illuminate recent efforts to better understand MDS/MPN, explore the diagnostic and management challenges, and define the areas where further progress is needed.

Unraveling the Diagnostic Challenges and Classification Updates in MDS/MPN

Complex network of blood cells and DNA strands representing MDS/MPN diagnostics.

MDS/MPN are defined by features of both MDS and MPN, making the diagnosis a challenge. The WHO diagnostic criteria are summarized in Table 1. The revised WHO 2016 classification retained the original MDS/MPN subtypes with further refinement and nomenclature (Table 1). For CMML, persistent monocytosis more than 3 months is adequate for diagnosis, even with a lack of morphologic dysplasia. The challenge is excluding other causes of monocytosis, cytogenetic abnormalities, and somatic mutations, which may help identifying clonal hematopoiesis, ruling out the reactive process. The coexistence of TET-2 and SRSF2 somatic mutations are suggested to be highly specific for CMML.

A clonal marker can be identified in 93% using next-generation sequencing on peripheral blood or bone marrow.

  • CMML (Chronic Myelomonocytic Leukemia): Diagnosed by persistent monocytosis (over 3 months). Subtypes (CMML-0, CMML-1, CMML-2) are classified based on blast percentages in the blood and bone marrow. Crucially, other causes of monocytosis must be ruled out, including infections and inflammatory conditions. Genetic markers can help confirm clonality.
  • aCML (Atypical Chronic Myeloid Leukemia): Diagnosed as BCR/ABL1 negative. Requires exclusion of CML and presence of granulocytic dysplasia.
  • RARS-T (Refractory Anemia with Ring Sideroblasts and Thrombocytosis): Now known as MDS/MPN-RS-T. Diagnosis requires a high platelet count and ring sideroblasts in the bone marrow. Common mutations include SF3B1, JAK2 and CALR.
  • JMML (Juvenile Myelomonocytic Leukemia): Typically diagnosed in children with persistent monocytosis, absence of BCR-ABL or PDGFR fusion genes, and specific genetic abnormalities.
  • MDS/MPN-U (Unclassifiable): Diagnosed when features of both MDS and MPN are present, but the condition doesn't fit into other defined categories. Requires careful exclusion of other possibilities.
MDS/MPN-U relies on clinicopathologic features, highlighting the difficulty of its diagnosis.2

Future Directions and the ABNL MARRO Study

The MDS/MPN International Working Group is actively working to advance therapies for MDS/MPN. Soon, they will begin the ABNL MARRO (A Novel therapy combinations in untreated MDS/MPN And Relapsed/Refractory Overlap Syndromes) basket trial. This trial will incorporate novel therapies with a DNA methyltransferase inhibition backbone for patients with previously treated or treatment-naïve MDS/MPN.

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This article is based on research published under:

DOI-LINK: 10.1016/j.clml.2018.11.019, Alternate LINK

Title: Challenges In Myelodysplastic/Myeloproliferative Neoplasms (Mds/Mpn)

Subject: Cancer Research

Journal: Clinical Lymphoma Myeloma and Leukemia

Publisher: Elsevier BV

Authors: Brianna N. Smith, Michael Savona, Rami S. Komrokji

Published: 2019-01-01

Everything You Need To Know

1

What are Myelodysplastic/Myeloproliferative Neoplasms (MDS/MPN), and why are they considered complex?

Myelodysplastic/Myeloproliferative Neoplasms (MDS/MPN) are a group of complex diseases that bridge Myelodysplastic Syndromes (MDS) and Myeloproliferative Neoplasms (MPN). MDS is characterized by ineffective hematopoiesis, while MPN involves proliferative features. The complexity arises because MDS/MPN share clinical, pathological, and molecular characteristics of both MDS and MPN, making diagnosis and treatment challenging. The WHO recognized this overlap in 2008, defining specific entities within this category. Understanding the genetic and molecular underpinnings requires advanced techniques like next-generation sequencing to identify clonal markers.

2

How has the classification and diagnosis of Chronic Myelomonocytic Leukemia (CMML) evolved, and what are the key diagnostic criteria?

The diagnosis of Chronic Myelomonocytic Leukemia (CMML) requires persistent monocytosis (more than 3 months), even without morphologic dysplasia. Subtypes (CMML-0, CMML-1, CMML-2) are classified based on blast percentages in the blood and bone marrow. A key challenge is excluding other causes of monocytosis, such as infections and inflammatory conditions. Genetic markers, like the coexistence of TET-2 and SRSF2 somatic mutations, can aid in confirming clonality. Next-generation sequencing on peripheral blood or bone marrow can identify a clonal marker in the majority of cases. The WHO 2016 classification retained the original MDS/MPN subtypes while providing further refinement and nomenclature.

3

What are the defining characteristics of Refractory Anemia with Ring Sideroblasts and Thrombocytosis (RARS-T), now known as MDS/MPN-RS-T, and what genetic mutations are commonly associated with it?

Refractory Anemia with Ring Sideroblasts and Thrombocytosis (RARS-T) is now known as MDS/MPN-RS-T. The diagnosis requires a high platelet count and the presence of ring sideroblasts in the bone marrow. Common mutations include SF3B1, JAK2, and CALR. The reclassification reflects a better understanding of its overlap between MDS and MPN features. It's crucial to differentiate MDS/MPN-RS-T from other similar conditions through comprehensive morphological and genetic evaluation.

4

What is the ABNL MARRO study, and how does it aim to advance therapies for MDS/MPN?

The ABNL MARRO (A Novel therapy combinations in untreated MDS/MPN And Relapsed/Refractory Overlap Syndromes) study is a basket trial initiated by the MDS/MPN International Working Group. It aims to advance therapies for MDS/MPN by incorporating novel therapies with a DNA methyltransferase inhibition backbone. The trial targets patients with previously treated or treatment-naïve MDS/MPN. This approach seeks to define the clinical benefits of specific therapies for this unique group of patients, addressing the need for more effective treatment strategies.

5

What makes MDS/MPN-U (Unclassifiable) challenging to diagnose, and why is it important to exclude other possibilities?

MDS/MPN-U (Unclassifiable) is challenging to diagnose because it relies on clinicopathologic features and doesn't fit into other defined MDS/MPN categories. It requires careful exclusion of other possibilities to ensure an accurate diagnosis. The condition presents features of both MDS and MPN, but lacks specific criteria for other subtypes. Its diagnosis underscores the complexity and heterogeneity within myeloid neoplasms. Further research and refined diagnostic criteria are needed to better classify and manage MDS/MPN-U.

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