DNA strands forming a Zebu cattle shape in a tropical background.

Decoding Cattle: How Understanding Indicine Genomes Can Boost Livestock Resilience

"Unlock the secrets of Bos indicus cattle: New research identifies key genetic variations that enhance immunity, heat tolerance, and disease resistance, paving the way for stronger, healthier herds."


Modern cattle breeds descend from aurochs and are generally classified into two subspecies: Bos taurus and Bos indicus. Bos indicus, often called indicine or zebu cattle, are known for their exceptional ability to thrive in tropical climates. This resilience stems from unique physiological traits, including superior heat tolerance, robust immunity, and resistance to various diseases.

MicroRNAs (miRNAs) are small, non-coding RNA molecules that play a crucial role in regulating gene expression. These tiny molecules influence a wide range of biological processes in animals, acting as key controllers in cellular and physiological functions. Variations in miRNA expression and sequence can significantly impact traits like disease resistance and immunity.

Recent advancements in genome sequencing have opened new avenues for understanding the genetic makeup of different cattle breeds. This article explores findings from a study focused on indicine cattle, utilizing advanced bioinformatics techniques to identify conserved miRNAs and predict their target genes. By comparing the indicine genome with that of taurine cattle, researchers have uncovered key genetic differences that contribute to the unique resilience of indicine breeds.

Unlocking Resilience: Key miRNA Findings in Indicine Cattle

DNA strands forming a Zebu cattle shape in a tropical background.

The study employed both de novo and homology-based in silico approaches to analyze the indicine genome and compare it to the taurine genome. Researchers successfully mapped 768 miRNAs from the taurine genome onto the indicine genome, identifying several key variations. These variations include differences in copy number, sequence polymorphisms, and the presence of novel miRNAs unique to indicine cattle.

Several interesting trends emerged from the analysis:

  • Increased miRNA Incidence: Certain chromosomes (chr5, chr7, chr8, chr12, and chr21) showed a higher concentration of miRNAs in the indicine genome.
  • Polycistronic miRNAs: Nineteen instances of polycistronic miRNA clusters were found within a 1-kilobase distance, indicating coordinated gene regulation.
  • Copy Number Variations (CNVs): Twelve miRNAs displayed differences in copy number between indicine and taurine cattle.
  • Mature Sequence Mutations: Eighteen miRNAs exhibited mutations in their mature sequences, with eight of these occurring in the critical seed region responsible for target recognition.
In addition to mapping known miRNAs, the study also identified 12 novel potential miRNAs located on the Y chromosome of indicine cattle. Furthermore, a new miRNA (bind-miR-1264) was discovered on the X chromosome. These novel miRNAs could play a significant role in the unique characteristics of indicine cattle.

Future Implications: Breeding for a Resilient Future

The identification of conserved and novel miRNAs in indicine cattle, along with the discovery of sequence variations, offers valuable insights into the genetic mechanisms underlying their resilience. This knowledge can be applied to develop targeted breeding strategies aimed at enhancing specific traits, such as immunity, heat tolerance, and disease resistance.

By selecting for specific miRNA profiles, breeders can potentially create hardier and more adaptable cattle breeds that are better equipped to withstand the challenges of changing environments and disease pressures. This is particularly important in tropical regions, where livestock production is often hampered by heat stress and disease outbreaks.

Further research is needed to fully elucidate the functional roles of the identified miRNAs and their target genes. However, this study provides a crucial foundation for future investigations into the molecular basis of resilience in indicine cattle and offers promising avenues for improving livestock management practices.

About this Article -

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This article is based on research published under:

DOI-LINK: 10.1371/journal.pone.0206154, Alternate LINK

Title: In Silico Identification Of Conserved Mirnas And Their Selective Target Gene Prediction In Indicine (Bos Indicus) Cattle

Subject: Multidisciplinary

Journal: PLOS ONE

Publisher: Public Library of Science (PLoS)

Authors: Quratulain Hanif, Muhammad Farooq, Imran Amin, Shahid Mansoor, Yi Zhang, Qaiser Mahmood Khan

Published: 2018-10-26

Everything You Need To Know

1

What are Bos indicus cattle, and why are they important?

Bos indicus cattle, also known as indicine or zebu cattle, are a subspecies of modern cattle known for their exceptional ability to thrive in tropical climates. This resilience comes from unique physiological traits, including superior heat tolerance, robust immunity, and resistance to various diseases. The article explores the genetic basis of this resilience by examining microRNA variations.

2

What are microRNAs, and why are they relevant to this research?

MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression. They play a crucial role in various biological processes in animals, influencing traits like disease resistance and immunity. Variations in miRNA expression and sequence can significantly impact these traits. The study focused on identifying these variations in Bos indicus to understand the genetic mechanisms behind their resilience to environmental challenges.

3

What key differences were found in the indicine genome compared to taurine cattle?

The study compared the indicine genome to that of taurine cattle. Researchers found key genetic differences. These included differences in copy number, sequence polymorphisms, and the presence of novel miRNAs unique to indicine cattle. Specific chromosomes showed a higher concentration of miRNAs. Certain miRNAs displayed differences in copy number. Mutations were found in the mature sequences of others. These findings are significant as they highlight the genetic variations that contribute to the unique resilience of Bos indicus.

4

How can this research be used to improve cattle breeding?

The identification of specific microRNA variations offers valuable insights into the genetic mechanisms underlying Bos indicus's resilience. This information can be applied to develop targeted breeding strategies. These strategies would enhance specific traits like immunity, heat tolerance, and disease resistance. By understanding the role of these miRNAs, scientists can potentially improve the health and productivity of cattle breeds.

5

What are some of the specific findings about miRNAs in Bos indicus?

Key findings include increased miRNA incidence on certain chromosomes, the discovery of polycistronic miRNA clusters, copy number variations in specific miRNAs, and mutations in mature sequences. Novel miRNAs were also identified, including those on the Y and X chromosomes. For example, the new miRNA bind-miR-1264 was discovered on the X chromosome. These variations and novel miRNAs are significant because they contribute to the unique characteristics and resilience of Bos indicus cattle, providing potential targets for breeding programs.

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