A steak divided into tender and tough halves, symbolizing consumer choice and scientific analysis.

Unlocking Tenderness: A Consumer's Guide to Beef Quality and Beta-Agonists

"Delve into the science behind beef tenderness and learn how beta-agonists like zilpaterol and ractopamine affect your steak's quality."


For years, the beef industry has been exploring innovative ways to enhance meat production. Among these methods, beta-adrenergic agonists (BAAs) have emerged as key players, promising improved performance and increased carcass yield. Zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH) are two prominent BAAs used in beef production. They're designed to help cattle gain weight more efficiently and produce leaner carcasses.

However, the use of these supplements isn't without its questions. Consumers and producers alike are keen to understand how these additives affect the final product—the steak on your plate. Does it impact tenderness, flavor, or overall eating experience? Recent research has been diving deep into these questions to provide clarity and inform consumer choices.

This guide will walk you through a comprehensive study that examines the effects of supplementing beef steers with ZH and RH, comparing them to steers raised without these BAAs. We'll explore how these supplements influence everything from the composition of the meat to its color, tenderness, and, most importantly, how consumers perceive the quality of the steak.

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The Consumer Stakes of Beef Tenderness

Research involving 734 consumers demonstrates that the beef industry could establish different tenderness threshold categories to meet varying consumer expectations, reflecting the significant economic weight of this quality trait. Tenderness management requires control and verification of specific processes throughout the beef chain, both before and after harvest, to influence the tenderness of the final product. Studies using a farm-to-fork continuum approach have applied machine learning methods including decision trees to properly group steaks by their tenderness potential, underscoring the complexity of predicting what consumers will accept as tender.

Measuring Tenderness: Methods and Their Limits

Tenderness is the most critical sensory trait related to overall beef palatability, yet its measurement remains difficult because tenderness gradients exist both within and among muscles, and the traditional shear force technique has inherent variation itself. For consumer-facing applications, beef cuts can be certified through third-party auditing activities under USDA standard specifications for tenderness marketing claims, requiring firms to adhere to recognized standard practices. Beyond objective measurement, practical guidance holds that tender cuts cook best with fast, high heat methods such as grilling or pan-frying, while tougher cuts benefit from different preparation approaches.

A Three-Decade Quest for Tenderness

The National Beef Tenderness Survey was initiated in 1990 by the National Cattlemen's Beef Association, a contractor to the Beef Checkoff, to quantify beef tenderness and identify areas for improvement across the industry. Groundbreaking analysis published in 2020 produced the world's most robust set of beef tenderness biomarkers and major molecular signatures, representing a milestone in understanding the biological basis of tenderness. A comprehensive history of tenderness research documents the key developments and sustainable improvements achieved in beef production over this period.

The Science of Supplements: Zilpaterol vs. Ractopamine

A steak divided into tender and tough halves, symbolizing consumer choice and scientific analysis.

A detailed study assigned 1,914 beef steers to one of three supplementation treatments: zilpaterol hydrochloride (ZH), ractopamine hydrochloride (RH), or no beta-agonist (CON). The goal was to determine the effects of these treatments on consumer eating quality. Strip loins were obtained and fabricated into steaks for various analyses, including Warner-Bratzler shear force (WBSF), slice shear force (SSF), and consumer assessments. Steaks were aged for either 14 or 21 days postmortem to observe changes over time.

The study meticulously analyzed various aspects of the beef, from its composition to its tenderness. Here's a breakdown of the key findings:

  • Protein Content: Steaks from steers fed ZH had more protein compared to those from steers fed CON or RH.
  • Shear Force: ZH steaks aged for 14 days required the most force to shear, while RH steaks were intermediate, and CON steaks had the lowest shear force values. However, after 21 days of aging, RH steaks exhibited the lowest shear force values.
  • Consumer Perception: In steaks aged 14 days, BAA supplementation affected tenderness, flavor, and overall liking, resulting in lower consumer scores for ZH compared to CON and RH. By 21 days, BAA only influenced tenderness and juiciness scores.
  • Quality Grade Impact: Quality grade significantly impacted all traits and acceptability in steaks aged 14 and 21 days. Premium Choice steaks typically scored higher than Low Choice or Select at 14 days.
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New Frontiers in Tenderness Science

Recent research indicates that DNA methylation may affect beef tenderness, suggesting that epigenetic mechanisms play a role in controlling this highly valued quality trait and opening new avenues for breeding and management decisions. Proteomic studies have advanced the path from protein profiling to biomarkers, with integrative approaches combining data from multiple platforms to identify molecular signatures underlying tenderness variation. The latest US National Beef Tenderness Survey, published in the Journal of Animal Science, has identified significant increases in consumer perceptions of beef palatability over the past decade, suggesting that industry efforts are yielding measurable improvements.

Challenges in Predicting and Ensuring Tenderness

Direct measurement of tenderness using instruments such as the Warner-Bratzler shear force test avoids the problems associated with using indirect indicators to predict expected tenderness, offering the opportunity to sort carcasses into tenderness categories. However, the shear force test itself is an imperfect proxy for the actual eating experience, since tenderness remains fundamentally a sensory trait that no single instrumental method fully captures. Proteomic research has identified proteins considered good biomarkers for both tenderness and intramuscular fat content, yet translating these laboratory findings into reliable, scalable industry applications remains an ongoing challenge.

Cut by Cut: How Tenderness Varies

Comparative investigations into beef tenderness determination have examined how preparation methods, measuring techniques, and sample location within the longissimus muscle all influence tenderness and cooking traits, demonstrating that results depend heavily on methodology. In head-to-head comparisons, sirloin and filet mignon differ substantially in tenderness, flavor intensity, price, and ideal cooking methods, with filet mignon generally considered the more tender option. Nutritional comparisons between fresh and frozen beef reveal that fresh beef generally contains slightly higher levels of certain nutrients, particularly vitamins B and C, which are sensitive to freezing temperatures.

These findings suggest that while ZH can increase protein content, it may initially compromise tenderness. However, aging the meat for a longer period can mitigate these differences. RH, on the other hand, shows a stronger response to aging, ultimately resulting in more tender steaks.

Making Informed Choices at the Meat Counter

Ultimately, the choice of whether to purchase beef from cattle supplemented with BAAs comes down to individual preferences. If you prioritize leaner meat and are willing to age your steaks a bit longer, ZH-supplemented beef might be a good option. If tenderness is your primary concern, RH-supplemented or non-supplemented beef could be more appealing. Keep in mind that quality grade also plays a significant role in the overall eating experience, so opting for Premium Choice can help ensure a satisfying meal, regardless of supplementation.

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Ranking Muscles and Understanding Biomarkers

Consumers, producers, and product development experts frequently seek tenderness rankings of various beef muscles, and scientific studies have addressed this by examining many muscles across many animals to produce reliable comparisons. Multiple regression analyses using 21 protein biomarkers as explanatory variables have been applied to explain overall tenderness, Warner-Bratzler shear force, and tenderness index traits across different muscles, revealing inverse relationships between certain biomarkers and measured tenderness. Decision tree learning tools have been developed for tenderness prediction by integrating rearing factors and carcass characteristics, though their accuracy depends on the breadth and quality of the underlying data.

Predicting Tenderness Before the First Cut

USDA Agricultural Research Service scientists are developing a system that predicts both beef and pork tenderness as well as color stability, representing a potential breakthrough for consistent quality assurance across the meat industry. Kansas State University meat scientists, supported by beef checkoff funding, have studied how fat content, muscle structure, and aging influence tenderness across three specific cuts—the striploin, tri-tip, and heel—finding that tenderness is very much driven by the individual cut. These efforts point toward a future where predictive tools could guide producers, processors, and consumers toward optimal tenderness outcomes.

Thirty Years of Progress Despite Headwinds

Despite challenges including drought, herd liquidation, fluctuating supply, and rising input costs, the beef industry has seen great improvements in overall quality and tenderness over the past 30 years according to the National Cattlemen's Beef Association. A USDA study has revealed that beef tenderness reflects interconnected metabolic systems that vary by muscle, reinforcing why single biomarkers often fall short of providing a complete picture. This systemic complexity means that achieving consistent tenderness requires coordinated efforts across the entire production chain rather than isolated interventions at any single point.

Feeding Practices and Tender Outcomes

Supplementation of ractopamine hydrochloride has consistently shown a small but negative impact on beef tenderness, and the combined effects of ractopamine and zinc feeding on muscle and meat quality are not yet fully understood. Research on specific cuts—the striploin, tri-tip, and heel—confirms that tenderness is very much driven by the individual cut, influenced by its fat content, muscle structure, and aging history. Teagasc research demonstrated how protein dynamics across different metabolic pathways are associated with the development of tenderness early post-mortem and during the aging process, offering practical insights for producers seeking to optimize eating quality through informed management decisions.

About this Article -

Written with AI assistance from published research, and reviewed by the Mystum team. See our About page for more information.

This article is based on research published under:

DOI-LINK: 10.2527/jas.2014-7840, Alternate LINK

Title: Comparative Effects Of Supplementing Beef Steers With Zilpaterol Hydrochloride, Ractopamine Hydrochloride, Or No Beta Agonist On Strip Loin Composition, Raw And Cooked Color Properties, Shear Force, And Consumer Assessment Of Steaks Aged For Fourteen Or Twenty-One Days Postmortem1,2

Subject: Genetics

Journal: Journal of Animal Science

Publisher: Oxford University Press (OUP)

Authors: A. J. Garmyn, J. C. Brooks, J. M. Hodgen, W. T. Nichols, J. P. Hutcheson, R. J. Rathmann, M. F. Miller

Published: 2014-08-01

Everything You Need To Know

1

What are zilpaterol hydrochloride and ractopamine hydrochloride, and how are they used in beef production?

Beta-adrenergic agonists like zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH) are used in beef production to help cattle gain weight more efficiently and produce leaner carcasses. Zilpaterol hydrochloride tends to increase protein content in the meat. Ractopamine hydrochloride shows a stronger response to aging, potentially resulting in more tender steaks. The use of these supplements aims to improve carcass yield, but their impact on tenderness and consumer perception can vary.

2

How do Warner-Bratzler shear force (WBSF) and slice shear force (SSF) relate to beef tenderness, and what did the study reveal about their values concerning zilpaterol hydrochloride and ractopamine hydrochloride?

Warner-Bratzler shear force (WBSF) and slice shear force (SSF) are methods used to measure the tenderness of beef. Lower shear force values typically indicate more tender meat, as less force is required to cut through it. In the study, steaks from steers fed zilpaterol hydrochloride initially required the most force to shear, while ractopamine hydrochloride steaks showed a better response to aging, resulting in lower shear force values over time. These measurements provide objective data on how different treatments affect meat tenderness.

3

How does the quality grade of beef influence consumer perception of traits like tenderness and flavor, and what role does it play alongside beta-agonists?

The study found that quality grade significantly impacted consumer perception of beef tenderness, flavor, and overall liking. Premium Choice steaks generally scored higher than Low Choice or Select steaks at 14 days of aging. This indicates that selecting a higher quality grade can improve the eating experience, regardless of whether the cattle were supplemented with beta-agonists like zilpaterol hydrochloride or ractopamine hydrochloride. However, quality grade is independent of beta-agonists.

4

In what ways does aging impact the tenderness of beef from cattle treated with beta-agonists like zilpaterol hydrochloride and ractopamine hydrochloride?

Aging beef affects tenderness differently depending on whether the cattle received beta-agonists. For instance, zilpaterol hydrochloride (ZH) supplemented steaks, which initially may be tougher, can become more tender with longer aging (e.g., 21 days). Ractopamine hydrochloride (RH) supplemented steaks showed a stronger response to aging resulting in more tender steaks after 21 days. This suggests that aging can mitigate some of the initial tenderness differences caused by these supplements.

5

What are the implications of using beta-agonists like zilpaterol hydrochloride and ractopamine hydrochloride on beef quality, and how should consumers make informed choices at the meat counter?

While zilpaterol hydrochloride (ZH) can increase protein content in beef, it may initially compromise tenderness. Ractopamine hydrochloride (RH) shows a stronger response to aging, potentially resulting in more tender steaks over time. Consumers should consider their priorities: If leaner meat is desired and they are willing to age the steaks longer, ZH-supplemented beef might be suitable. If tenderness is the main concern, RH-supplemented or non-supplemented beef could be more appealing. It's also crucial to consider the quality grade, as Premium Choice generally offers a better eating experience.

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