Traveler examining malaria parasites with magnifying glass

Malaria Detection: A New LAMP Test for Travelers

Nico Varela in Health & Wellness August 2025 • 4 min read.

"Discover how a commercial LAMP test offers a faster, more accurate way to rule out malaria in returning travelers, reducing healthcare costs and improving patient care."

Malaria, a disease affecting millions worldwide, demands accurate and timely diagnosis. Traditionally, healthcare providers have relied on microscopy and rapid diagnostic tests (RDTs). However, these methods have limitations, especially in non-endemic areas like North America, where the vast majority of tests come back negative.

A new study published in Open Forum Infectious Diseases explores the potential of loop-mediated isothermal amplification (LAMP) assays to improve malaria detection in returning travelers. This prospective diagnostic trial, conducted between June 2017 and January 2018, compares the Meridian illumigene Malaria assay with conventional microscopy and RDTs, offering a promising alternative for ruling out malaria with greater accuracy and efficiency.

The study enrolled 298 patients presenting with malaria-like symptoms. Researchers sought to evaluate the clinical performance of the LAMP test against microscopy and RDTs. The findings highlight the LAMP assay's potential to transform malaria diagnostics, offering a faster, more sensitive method for healthcare providers.

LAMP Assay: A New Diagnostic Tool

Traveler examining malaria parasites with magnifying glass

The study’s results indicate that the LAMP assay has a high sensitivity and specificity compared to traditional microscopy. In the prospective arm of the study, LAMP demonstrated a sensitivity of 98.1% (95% CI, 90.0%-100%) and a specificity of 97.6% (95% CI, 95.2%-99.1%) against microscopy. After discrepant resolution using real-time polymerase chain reaction (PCR), the LAMP test achieved 100% sensitivity and specificity.

In contrast, RDTs showed a sensitivity of 83.3% (95% CI, 58.6%–96.4%) and a specificity of 96.2% (95% CI, 93.2%-98.1%) after discrepant resolution. These findings suggest that LAMP assay could offer a more reliable method for excluding malaria in returning travelers.

Improved Sensitivity: LAMP showed higher sensitivity than RDTs.
High Specificity: LAMP maintained high specificity, minimizing false positives.
Reduced Repeat Testing: LAMP’s accuracy could reduce the need for repeat testing.
Faster Results: LAMP offers quicker turnaround times.

Researchers also investigated the cost-effectiveness of implementing LAMP in a diagnostic algorithm. The analysis suggested potential savings of up to USD$13 per specimen. This is due to reduced labor costs and fewer repeat tests. Integrating LAMP into routine diagnostic procedures can optimize resource allocation and improve overall efficiency.

Implications for Malaria Diagnostics

The study underscores the limitations of traditional malaria diagnostic methods and highlights the potential of the LAMP assay to improve diagnostic accuracy and efficiency. By implementing LAMP, healthcare providers can reduce the need for repeat testing, lower healthcare costs, and provide faster results. This approach ultimately improves patient care and supports malaria elimination efforts in non-endemic regions.

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This article is based on research published under:

DOI-LINK: 10.1093/ofid/ofy260, Alternate LINK

Title: Clinical Validation Of A Commercial Lamp Test For Ruling Out Malaria In Returning Travelers: A Prospective Diagnostic Trial

Subject: Infectious Diseases

Journal: Open Forum Infectious Diseases

Publisher: Oxford University Press (OUP)

Authors: James Cheaveau, Hong Nguyen, Barbara Chow, Dewdunee Marasinghe, Abu Naser Mohon, Hong Yuan, Gisele Viana, Donelly Van Schalkwyk, Deirdre Church, Wilson Chan, Dylan R Pillai

Published: 2018-10-12

Everything You Need To Know

What is the loop-mediated isothermal amplification (LAMP) assay and how does it aid in malaria detection?

The loop-mediated isothermal amplification (LAMP) assay is a molecular diagnostic tool used to detect the presence of malaria parasites. It amplifies the DNA of the parasite, making it easier to detect even when the parasite levels are low. In a study, the Meridian illumigene Malaria assay was evaluated, showing high sensitivity and specificity compared to traditional methods like microscopy and rapid diagnostic tests (RDTs). This makes LAMP particularly useful for rapidly and accurately ruling out malaria in returning travelers.

How does the accuracy of the loop-mediated isothermal amplification (LAMP) assay compare to traditional malaria diagnostic methods like microscopy and rapid diagnostic tests (RDTs)?

The study compared the loop-mediated isothermal amplification (LAMP) assay (specifically the Meridian illumigene Malaria assay) to traditional methods, namely microscopy and rapid diagnostic tests (RDTs). The LAMP assay demonstrated superior sensitivity (98.1% vs 83.3% for RDTs) and high specificity, indicating it's more reliable for excluding malaria. While microscopy is a standard, it can be less sensitive, and RDTs, while quick, may miss low-level infections. LAMP’s accuracy also reduces the need for repeat testing, making it a more efficient option.

What are the potential benefits of using the loop-mediated isothermal amplification (LAMP) assay for malaria diagnosis in terms of cost and patient care?

Using the loop-mediated isothermal amplification (LAMP) assay can lead to several improvements. Firstly, its higher sensitivity and specificity mean more accurate diagnoses. Secondly, the reduced need for repeat testing can lower healthcare costs, potentially saving up to USD$13 per specimen. Finally, the faster turnaround times of the Meridian illumigene Malaria assay allow for quicker clinical decision-making and improved patient care, especially crucial in non-endemic areas where malaria might not be the first consideration.

What are some limitations or challenges associated with using the loop-mediated isothermal amplification (LAMP) assay that were not addressed?

While the study focuses on the loop-mediated isothermal amplification (LAMP) assay, it doesn't delve into specific challenges like the initial investment in equipment or training for healthcare staff. Also, while LAMP shows great promise, its widespread adoption may face hurdles in resource-limited settings due to cost considerations. Further research might explore integrating LAMP with other diagnostic tools for a comprehensive approach. Additionally, understanding the performance of LAMP against different Plasmodium species and strains could enhance its utility in diverse geographical regions.

What are the broader implications of adopting the loop-mediated isothermal amplification (LAMP) assay in the context of malaria diagnostics and public health?

The study indicates that the loop-mediated isothermal amplification (LAMP) assay, particularly the Meridian illumigene Malaria assay, could be a transformative tool in malaria diagnostics, especially in non-endemic regions. Its high sensitivity and specificity mean fewer false negatives, ensuring travelers receive accurate diagnoses and appropriate treatment. The potential cost savings from reduced repeat testing could free up resources for other healthcare needs. By implementing LAMP, healthcare providers can enhance diagnostic accuracy, improve patient outcomes, and support malaria elimination efforts more effectively.