Citric acid molecules battling Aspergillus mold.

Aflatoxin Busters: Can Food Additives Really Clean Up Distillers Grains?

"Discover how common food additives like citric acid could be a game-changer in reducing aflatoxin contamination in distillers grains, making animal feed safer."


Distillers wet grains (DWG) and condensed distillers solubles (CDS)—byproducts of ethanol production—are valuable components of animal feed. Rich in protein, fat, and fiber, they offer a concentrated source of nutrients. Yet, this concentration also brings a hidden risk: mycotoxins, particularly aflatoxins, which can contaminate these grains and pose a significant threat to animal health.

Aflatoxins, produced by Aspergillus molds, are potent toxins and carcinogens. If contaminated corn is used in ethanol production, aflatoxins can end up in DWG and CDS, sometimes at levels exceeding safety limits. Current regulations set maximum allowable aflatoxin levels in animal feeds, but what happens when these levels are breached? The industry urgently needs effective and safe methods to reduce aflatoxin contamination in distillers grains.

This article dives into a promising solution: using food additives to degrade aflatoxins in DWG and CDS. We'll explore research evaluating the effectiveness of additives like sodium bisulfite, sodium hypochlorite, citric acid, and ammonium persulfate. Discover how these common substances can potentially detoxify distillers grains, paving the way for safer animal feed and a more secure supply chain.

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How Aflatoxin Research Took Shape

Aflatoxin research developed through a collective effort involving experts in veterinary medicine, animal nutrition, toxicology, chemistry, and mycology. The toxic substance was named “aflatoxin,” and it was later found to comprise several closely related toxic components. A 2021 review surveys the history and principal milestones of aflatoxin research, its toxicity, and mitigation approaches, with particular emphasis on biocontrol. The review also describes aflatoxins as a concern for health and food security, with social, economic, and political effects.

The Power of Food Additives: Finding the Right Aflatoxin Fighter

Citric acid molecules battling Aspergillus mold.

Researchers tested four food additives to see how well they could break down aflatoxins in DWG and CDS. The goal? To find a practical, safe, and effective solution that ethanol plants could easily implement. Here's a breakdown of what they discovered:

Sodium Bisulfite: This additive, often used as a preservative, didn't show much promise in reducing aflatoxin levels in either DWG or CDS. While some studies have suggested its effectiveness in corn, the one-hour treatment time in this experiment may have been too short to yield significant results.

  • Sodium Hypochlorite: Known for its strong oxidizing power (think bleach), sodium hypochlorite was the most effective at degrading aflatoxins. However, it came with a significant drawback: it bleached the grains and left an unpleasant odor, making it unsuitable for practical use.
  • Citric Acid and Ammonium Persulfate: These additives showed moderate success, reducing aflatoxin levels by 31% to 51%. Citric acid stood out as the most promising option due to its safety and widespread use in the food industry. It's also classified as generally recognized as safe (GRAS) by the FDA.
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A Range of Emerging and Established Approaches

The listed research covers several distinct approaches to aflatoxin management. A cited study by Shi et al. (2017) determined the relative effectiveness of food additives in degrading aflatoxin in distillers’ wet grains and condensed distillers soluble. Another source describes citric acid as a food additive that can serve antioxidative and aflatoxin-clearance purposes. A review reports that ammonium persulfate was associated with a 31–51% decrease in AFB1 contents, while a separate review describes cold plasma as generating reactive oxygen species that degrade aflatoxins on food surfaces without compromising nutritional integrity or safety.

Detoxification Methods Have Trade-Offs

A 2018 review says that a fully systematic discussion of aflatoxin detoxification methods was still unavailable, and it summarizes the merits and demerits of traditional and newer strategies. A 2026 review distinguishes adsorption-type from inhibition-type strategies and discusses their advantages and limitations across food and feed matrices. Another review frames cold plasma as a potential treatment whose effectiveness for mitigating aflatoxin risks is being evaluated. A 2026 review describes yeast-based detoxification as promising, with adsorption by cell-wall components and enzymatic biodegradation among the mechanisms under study.

Choosing a Method Depends on the Food Matrix

A comparative guide says that selecting an aflatoxin B1 (AFB1) detoxification method depends on the food matrix, contamination level, and desired preservation of nutritional and sensory properties. It describes its tables as summarizing quantitative efficiency data for physical, chemical, and biological methods. A 2026 systematic review presents biological detoxification as a promising alternative, motivated by the toxicity, carcinogenicity, and persistence of AFB1 in the food chain. The available source descriptions do not provide specific comparative efficiency figures.

Based on these initial findings, citric acid emerged as the frontrunner for further investigation. The key question became: could increasing the concentration of citric acid and extending the treatment time further enhance its aflatoxin-busting capabilities?

Citric Acid: The Future of Aflatoxin Control in Distillers Grains?

This research highlights the potential of citric acid as a safe and effective tool for managing aflatoxin contamination in distillers grains. By optimizing the concentration and treatment time, ethanol producers could significantly reduce aflatoxin levels, ensuring the safety and marketability of their products.

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Sustainable Biological Detoxification

A 2026 systematic review identifies AFB1 contamination of food and feed as a continuing concern because of its toxicity, carcinogenicity, and persistence in the food chain. It reports that environmental and climatic pressures continue to favor contamination by aflatoxigenic fungi. The review presents effective and sustainable detoxification strategies as a continuing need and biological detoxification as a promising area. These points indicate a research direction, rather than establishing that a particular biological method is already proven for every food or feed setting.

Adapting Decontamination to Real Foods

A 2024 review highlights smart packaging and artificial intelligence (AI) among technologies being considered for aflatoxin decontamination in foods. It suggests integrating multiple strategies to improve efficiency and adaptability across different food matrices. The source presents this integration as a recommendation for achieving more adaptable decontamination, rather than reporting a quantified outcome.

While further research is needed to fine-tune the application of citric acid in industrial settings, the findings offer a promising path forward. The method is easy to implement, cost-effective and safe. This positions it as a valuable strategy for ethanol plants looking to mitigate the risks associated with aflatoxins in DWG and CDS.

Ultimately, adopting such detoxification technologies benefits the entire supply chain, from producers to livestock farmers, and consumers. By ensuring safer animal feed, we can promote healthier livestock and a more secure food supply for everyone.

How the reported reductions compare across materials

The reported effect of citric acid varies with the material and treatment: a corn-based model system treated with citric acid and heat had a different reported reduction from the results in distillers grains and solubles. These figures are not a like-for-like comparison because the materials and treatment conditions differ.[1] [2]

Other reported approaches include physical treatments and chemical agents, while a review cautions that effectiveness depends on the material and application conditions.[3] [4]

Citric acid results alongside heating alone

  • >86% degradationIn the corn-based model system, the cited combination was 1% citric acid with treatment at 90 °C for 1 hour, and the reported aflatoxin B1 degradation exceeded 86%.[1]

What the treatment comparisons leave unresolved

  • How much of the reductions in DWG and CDS came from citric acid rather than heating, given that the reported heating durations differ?[2]
  • Would the reported outcomes hold across other food and feed materials, when treatment efficiency is said to depend on the matrix and application conditions?[4]

About this Article -

Written with AI assistance from published research, and reviewed by the Mystum team. See our About page for more information.

This article is based on research published under:

DOI-LINK: 10.4315/0362-028x.jfp-16-092, Alternate LINK

Title: Determination Of The Relative Effectiveness Of Four Food Additives In Degrading Aflatoxin In Distillers Wet Grains And Condensed Distillers Solubles

Subject: Microbiology

Journal: Journal of Food Protection

Publisher: International Association for Food Protection

Authors: Hu Shi, Richard L. Stroshine, Klein Ileleji

Published: 2016-12-21

Everything You Need To Know

1

What are aflatoxins, and why are they a concern?

Aflatoxins are potent toxins and carcinogens produced by Aspergillus molds. Their presence in Distillers wet grains (DWG) and condensed distillers solubles (CDS), which are byproducts of ethanol production, poses a significant threat to animal health. These toxins can contaminate these grains if the corn used in ethanol production is contaminated, potentially exceeding safety limits set by regulations. The industry needs safe methods to reduce aflatoxin contamination because of these risks.

2

What are Distillers wet grains (DWG) and condensed distillers solubles (CDS), and why are they important?

Distillers wet grains (DWG) and condensed distillers solubles (CDS) are byproducts of ethanol production. They are valuable components of animal feed. They are rich in protein, fat, and fiber, providing a concentrated source of nutrients for livestock. The significance lies in their nutritional value. However, they can be sources of contamination by mycotoxins, specifically aflatoxins, that can affect the animals' health.

3

How effective is citric acid in managing aflatoxins?

Citric acid showed moderate success in reducing aflatoxin levels in Distillers wet grains (DWG) and condensed distillers solubles (CDS) by 31% to 51%. Its advantage is that it's considered safe and is widely used in the food industry. It's also classified as generally recognized as safe (GRAS) by the FDA. This makes citric acid a promising option for further investigation.

4

What were the drawbacks of using sodium hypochlorite?

Sodium hypochlorite, known for its strong oxidizing power, effectively degraded aflatoxins, but it bleached the grains and left an unpleasant odor. This makes it unsuitable for practical use in animal feed. The goal is to find a balance between effectiveness and practicality in the application of food additives. The drawback of Sodium Hypochlorite is a significant factor in ruling out its use.

5

What was the overall goal of testing food additives?

Food additives like sodium bisulfite, sodium hypochlorite, citric acid, and ammonium persulfate were tested for their ability to break down aflatoxins in Distillers wet grains (DWG) and condensed distillers solubles (CDS). The aim was to find a solution that ethanol plants could easily implement. The research aimed to improve the safety of animal feed and secure the supply chain by reducing aflatoxin contamination using these additives.

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